Wednesday, October 21, 2020
Home Life Style Health & Fitness IISc Researchers Identify Cancer Cells Using a 3D Tumour Model, Nanomotors

IISc Researchers Identify Cancer Cells Using a 3D Tumour Model, Nanomotors

By N. B. Hombal

An interdisciplinary team of researchers from the Indian Institute of Science (IISc) has made a breakthrough in the field of identifying cancer cells by using a 3D tumour model and magnetically-driven nanomotors to probe the microenvironment of these cells.

The team consists of researchers from the Centre for Nano Science and Engineering (CeNSE) and Department of Molecular Reproduction, Development and Genetics (MRDG).

“What came as a beautiful surprise was that within such a milieu, we found that aggressive cancer cells ended up remodelling their surroundings by making them stickier, and richer in specific charged sugars,” Assistant Professor at MRDG and one of the senior authors Ramray Bhat observed.

Follow NewsGram on Quora Space to get answers to all your questions.

“This charging can potentially be used to target and kill tiny populations of cancer cells hidden among their normal counterparts, for which we are extending these studies to living animals,”

he added.

In their work, published in Angewandte Chemie, the team has steered helical nanomotors remotely via an external magnetic field through the tumour model to sense, map and quantify changes in the cellular environment.

“We believe these findings will find use as targeting strategies in future in-vivo applications, in quantifications of cancer aggressions and as biophysical probes to study the extracellular environment of cancer,”

the team concluded in the journal.

IISc scientists develop technique to study cancer cells
While a note released by the IISc (above) claimed that this study also highlights a new way of targeting cancer cells. Pinterest

“The model comprises both healthy and cancer cells embedded within a reconstituted basement membrane matrix, and mimics the breast cancer environment,” the paper published Angewandete Chemie stated. While a note released by the IISc claimed that this study also highlights a new way of targeting cancer cells by maneuvering nanomotors inside a tumour and waiting for them to localise in the vicinity of the cancerous site.

“We tried driving the nanomotors toward cancer cells in a tumour model and observed them getting stuck to the matrix near cancer cells, but this was not observed near normal cells,” the co-first author and PhD student at the CeNSE Debayan Dasgupta said.

Want to read more in Hindi? Checkout: बर्फ के पानी से बुझाते थे प्यास, अब जा कर मिली राहत

The IISc note further claimed that the extracellular matrix (ECM) is a complex 3D network of proteins and carbohydrates secreted by living cells into their neighbourhood.

“However, when cancer cells secrete fresh material into the ECM, it disrupts the chemical and physical composition of the native ECM surrounding healthy cells, degrading the local environment.”

Therefore, understanding how the cellular microenvironment is altered due to cancer cells and measuring these changes quantitatively could be vital in understanding the progression of cancer,” Dasgupta disclosed.

In the current study, the researchers discovered that as the nanomotors approached the cancer cell membrane, they stuck to the matrix more strongly than they would to normal cells. To measure how strongly the nanomotors bound to the matrix, the team calculated the magnetic field strength required to overcome the adhesive force, and move forward.

“This means that the cancer cells are doing something. So, we did some measurements and discovered that the adhesive force depended on the type of cells, the strength of interaction and also which side of the cell the nanomotor approached,”

Associate Professor at CeNSE and one of the senior authors Ambarish Ghosh explained.

“In the end, we really ended up discovering a physical property of an important biological environment,” he said.

IISc scientists develop technique to study cancer cells
In the current study, the researchers discovered that as the nanomotors approached the cancer cell membrane. Pinterest

The reason why the nanomotors appear to stick to the cancer cells better is their charged ECM. This may be due to the presence of 2, 3-linked sialic acid, a sugar-conjugated molecule which confers a negative charge on the cancer cell environment, the researchers found. They visualised the distribution of these sugars using fluorescent markers and found that sialic acids were distributed up to 40 micrometres from the cancer cell surface — the same distance until which the nanomotors experienced strong adhesion.

To counter this adhesive effect, the team coated the nanomotors with Perfluorooctyltriethoxysilane (PFO) which shielded them from the charged environment.

Also Read: It’s Time to Create Vibrant Cocktails with Simplest of Ingredients!

“The coated nanomotors did not stick to the matrix near the cancer cells, whereas the uncoated motors clung to the matrix, confirming the fact that the negatively charged cancer microenvironment interacts with the incoming nanomotors, rendering them immobile,” the paper published Angewandete Chemie read.

Prof. Ghosh further added that they have demonstrated how investigation of maneuverability of the nanomotors can be used to spatially differentiate between cancerous and non-cancerous cell niches that were engineered to coexist within tissue-like environment. (IANS)

STAY CONNECTED

19,120FansLike
362FollowersFollow
1,782FollowersFollow

Most Popular

Air Pollution Causes Food Delivery Leading to Plastic Waste

When the air quality outside is bad, office workers are more likely to order food than go out for lunch, which in turn increases...

Virus Can Reinfect COVID Patients When Antibodies Start Depleting

Patients who have recovered from COVID-19 can again get infected by the virus once the antibodies of the viral disease start depleting, the Indian...

International Chef Day a Low-Key Event Due to Pandemic

The International Chef Day is observed on October 20, but this year, the celebrations here were muted as chefs are facing a torrid time...

Coffee Before Breakfast May Increase Diabetes Risk

If you're taking coffee before breakfast, read this carefully. Researchers have found that drinking coffee first thing can have a negative effect on blood...

India’s Nutrition Experts on Supporting Public Health Delivery

Indias public health and nutrition efforts are differentiated by an enterprising early start going as far back as 1975 with the beginning of Anganwadi...

‘Fashion Reboot’- A Collection Focusing Gen Z

The final day of the digital edition of Lotus Make-up India Fashion Week, Spring/Summer 2021 on Sunday opened a film presentation that showcased a...

Disorder in Sleep Cycle Due to Excessive Use of Cellphones

Cell phones have become integral to function for nearly everyone. While it has brought a lot of tasks to our fingertips, cell phones also...

Higher Mortality Risk for COVID Patients with Kidney Diseases

Researchers, including one of Indian-origin, have found that there is a much higher risk of mortality faced by COVID-19 patients in intensive care, who...

Recent Comments