Researchers at Indian Institute of Technology Madras (IIT-M) have found that subjecting cancer cells to microgravity results in the formation of giant cancer cells with stem cell properties.
In a statement issued here on Tuesday, IIT-M said these cells can conceivably be used for cancer research and drug development.
Stem cells are difficult to isolate and grow. Cancer stem cells (CSCs) generally make up just one per cent to three per cent of all cells in a tumour.
Research is being conducted all over the world to extract and culture CSCs for cancer understanding and drug development, the statement said.
The research was led by Professor Rama S. Verma of the Stem Cell and Molecular Biology Laboratory, Bhupat, and Jyoti Mehta School of Biosciences, Department of Biotechnology, IIT-M.
“We have shown that simulated microgravity can be used for development of stem cell structures for drug testing, instead of animal models. CSCs are important in cancer research because they not only instigate formation of tumours, but are also involved in recurrence of tumours after cancer treatment,” Verma was quoted as saying in the statement.
He said the stem cells obtained using microgravity can also be used to understand the nature of the cancer cells, their proliferation and cell death pathways, which in turn can help in identification of target zones for drug development.
In an earlier study, the IIT Madras team had found that colorectal cancer cells died under simulated microgravity but once the microgravity condition was removed, they resurrected.
Heart Rate gets altered in space but return to normal within 10 days on Earth, say researchers who examined cell-level cardiac function and gene expression in human heart cells cultured aboard the International Space Station (ISS) for 5.5 weeks.
Exposure to microgravity altered the expression of thousands of genes, but largely normal patterns of gene expression reappeared within 10 days after returning to Earth, according to the study published in the journal Stem Cell Reports.
“We’re surprised about how quickly human heart muscle cells are able to adapt to the environment in which they are placed, including microgravity,” said senior study author Joseph C. Wu from Stanford University.
These studies may provide insight into cellular mechanisms that could benefit astronaut health during long-duration spaceflight, or potentially lay the foundation for new insights into improving heart health on Earth.
Past studies have shown that spaceflight induces physiological changes in cardiac function, including reduced heart rate, lowered arterial pressure, and increased cardiac output.
But to date, most cardiovascular microgravity physiology studies have been conducted either in non-human models or at tissue, organ, or systemic levels.
Relatively little is known about the role of microgravity in influencing human cardiac function at the cellular level.
To address this question, the research team studied human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). They generated hiPSC lines from three individuals by reprogramming blood cells, and then differentiated them into heart cells.
Beating heart cells were then sent to the ISS aboard a SpaceX spacecraft as part of a commercial resupply service mission.
Simultaneously, ground control heart cells were cultured on Earth for comparison purposes.
Upon return to Earth, space-flown heart cells showed normal structure and morphology. However, they did adapt by modifying their beating pattern and calcium recycling patterns.
In addition, the researchers performed RNA sequencing of heart cells harvested at 4.5 weeks aboard the ISS, and 10 days after returning to Earth.
These results showed that 2,635 genes were differentially expressed among flight, post-flight, and ground control samples.
Most notably, gene pathways related to mitochondrial function were expressed more in space-flown heart cells.
A comparison of the samples revealed that heart cells adopt a unique gene expression pattern during spaceflight, which reverts to one that is similar to groundside controls upon return to normal gravity, the study noted.
According to Wu, limitations of the study include its short duration and the use of 2D cell culture.
In future studies, the researchers plan to examine the effects of spaceflight and microgravity using more physiologically relevant hiPSC-derived 3D heart tissues with various cell types, including blood vessel cells.